Hotspot

trnM-CAU

LSC · 40115-40189 · 75 bp

Back to Mimusops

Pi 0.0133

MarkerSeek score 60.20

Primer available yes

Label trnM-CAU

Feature Metadata

label_name: trnM-CAU
feature_type: tRNA
region: LSC
length_bp: 75
variable_sites: 1
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU_p1	AGTGTAGTGGTTCTACCCCC	AGAGGCCCCTTTACCATTCT	55.0	50.0	58.1	58.0	292-294 bp	1.0	79.8
trnM-CAU_p2	AGTGTAGTGGTTCTACCCCC	GAGGCCCCTTTACCATTCTG	55.0	55.0	58.1	57.9	291-293 bp	1.0	79.4
trnM-CAU_p3	AGTGTAGTGGTTCTACCCCC	TACAGATACGGTAGAGGGGC	55.0	55.0	58.1	57.7	249-251 bp	1.0	77.6
trnM-CAU_p4	AGTGTAGTGGTTCTACCCCC	GGCGCATTCAATCCTTGTTT	55.0	45.0	58.1	57.6	232-234 bp	1.0	77.0
trnM-CAU_p5	AGTGTAGTGGTTCTACCCCC	CGATGATCATAGAGGCCCCT	55.0	55.0	58.1	58.4	302-304 bp	1.0	76.4