Hotspot

rpl22-rps19

LSC · 88569-88613 · 45 bp

Back to Mimusops

Pi 0.0222

MarkerSeek score 65.30

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 45
variable_sites: 1
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGGAACGTCCACGAATT	AAATTTCCGAGGACATGCGA	50.0	45.0	58.0	57.9	209 bp	1.0	79.4
rpl22-rps19_p2	CGTAGGAACGTCCACGAATT	AATTTCCGAGGACATGCGAA	50.0	45.0	58.0	57.9	208 bp	1.0	79.4
rpl22-rps19_p3	CGTAGGAACGTCCACGAATT	ATTTCCGAGGACATGCGAAA	50.0	45.0	58.0	57.9	207 bp	1.0	79.4
rpl22-rps19_p4	ACTTTGTGTGCAGACATCGA	AATTTCCGAGGACATGCGAA	45.0	45.0	57.8	57.9	170 bp	1.0	77.7
rpl22-rps19_p5	ACTTTGTGTGCAGACATCGA	ATTTCCGAGGACATGCGAAA	45.0	45.0	57.8	57.9	169 bp	1.0	77.7