Hotspot

accD-psaI

LSC · 62510-63429 · 920 bp

Back to Mimusops

Pi 0.00109

MarkerSeek score 60.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 920
variable_sites: 1
pi: 0.00109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	1028-1034 bp	1.0	75.2
accD-psaI_p2	CAGTTCCACGCTTTCTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	1029-1035 bp	1.0	74.8
accD-psaI_p3	AGTTCCACGCTTTCTTTCCT	AAGGCACAAAAATAGCGGGA	45.0	45.0	57.4	58.1	995-1001 bp	1.0	74.8
accD-psaI_p4	AGTTCCACGCTTTCTTTCCT	AGGCACAAAAATAGCGGGAA	45.0	45.0	57.4	58.1	994-1000 bp	1.0	74.8
accD-psaI_p5	AGTTCCACGCTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.4	57.9	1034-1040 bp	1.0	74.6