Hotspot

rpoB-trnC-GCA

LSC · 28726-29947 · 1222 bp

Back to Mimusops

Pi 0.00246

MarkerSeek score 60.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 3
pi: 0.00246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCCGTTAAGCCCTGA	GCAGAACAGAATGGGGGATT	50.0	50.0	57.5	57.9	1434-1441 bp	1.0	75.3
rpoB-trnC-GCA_p2	AGTTCTTCCGTTAAGCCCTG	GCAGAACAGAATGGGGGATT	50.0	50.0	57.5	57.9	1435-1442 bp	1.0	75.2
rpoB-trnC-GCA_p3	TTCATTTTCATCCCGGAGCA	GCAGAACAGAATGGGGGATT	45.0	50.0	57.5	57.9	1352-1359 bp	1.0	75.0
rpoB-trnC-GCA_p4	GTTCTTCCGTTAAGCCCTGA	GAACAGAATGGGGGATTCCG	50.0	55.0	57.5	58.3	1431-1438 bp	1.0	73.9
rpoB-trnC-GCA_p5	GTTCTTCCGTTAAGCCCTGA	TTATTGATCAGGCGACACCC	50.0	50.0	57.5	57.7	1405-1412 bp	1.0	73.9