Hotspot

atpH-atpI

LSC · 15317-16454 · 1138 bp

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Pi 0.00352

MarkerSeek score 60.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 4
pi: 0.00352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1226 bp	1.0	75.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	AGGTGAATCTATGGAGGGCC	45.0	55.0	58.0	58.6	1198 bp	1.0	75.5
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1282 bp	1.0	75.3
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	AGGTGAATCTATGGAGGGCC	50.0	55.0	57.9	58.6	1254 bp	1.0	74.9
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1227 bp	1.0	74.7