Hotspot

rps16-trnQ-UUG

LSC · 6457-8491 · 2035 bp

Back to Mimusops

Pi 0.00304

MarkerSeek score 53.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2035
variable_sites: 6
pi: 0.00304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	2093-2115 bp	1.0	81.9
rps16-trnQ-UUG_p2	TCCTGTCTTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	2097-2119 bp	1.0	80.8
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	2086-2108 bp	1.0	80.8
rps16-trnQ-UUG_p4	CCACAACGGATCCTGTCTTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	2107-2129 bp	1.0	80.4
rps16-trnQ-UUG_p5	GTCTTTCAAGTCGCACGTTG	AAATTTAAATGGGGCGTGGC	50.0	45.0	58.0	57.6	2150-2172 bp	1.0	79.2