Hotspot

ndhA

SSC · 77580-80236 · 2657 bp

Pi 0.0421

MarkerSeek score 74.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2657
variable_sites: 189
pi: 0.0421
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GATTTGCGTGGGCTAAGGTA	TGGAGATGGAAAATTCGCCC	50.0	50.0	58.0	58.2	2856-2975 bp	1.0	87.3
ndhA_p2	TTGCGTGGGCTAAGGTAATC	TGGAGATGGAAAATTCGCCC	50.0	50.0	58.0	58.2	2853-2972 bp	1.0	87.3
ndhA_p3	TGCAGCTCGTACTGTTTGTT	TGGAGATGGAAAATTCGCCC	45.0	50.0	58.1	58.2	2810-2929 bp	1.0	87.2
ndhA_p4	TGCAGCTCGTACTGTTTGTT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	58.1	58.0	2992-2995 bp	0.5	66.1
ndhA_p5	TTGCAGCTCGTACTGTTTGT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	58.1	58.0	2993-2996 bp	0.5	66.1