Hotspot

ccsA-ndhD

SSC · 72719-73527 · 809 bp

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Pi 0.136
MarkerSeek score 76.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
809
variable_sites
141
pi
0.136
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.73
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACCCAAGGCTTTGCTACTTC TGGGCCCCGAGAGTTATTTA 50.0 50.0 58.1 58.1 1339-1602 bp 0.5 67.8
ccsA-ndhD_p2 ACCCAAGGCTTTGCTACTTC ATCGGTTGACAAGGTCGAAG 50.0 50.0 58.1 57.9 1518 bp 0.25 48.6
ccsA-ndhD_p3 AATGAGGCCTGGGGATCATA ATCGGTTGACAAGGTCGAAG 50.0 50.0 57.9 57.9 1098 bp 0.25 48.2
ccsA-ndhD_p4 TAATGAGGCCTGGGGATCAT ATCGGTTGACAAGGTCGAAG 50.0 50.0 57.9 57.9 1099 bp 0.25 48.2
ccsA-ndhD_p5 TGAGGCCTGGGGATCATATT ATCGGTTGACAAGGTCGAAG 50.0 50.0 57.9 57.9 1096 bp 0.25 48.2