Hotspot

rpl32-trnL-UAG

SSC · 71099-71556 · 458 bp

Back to Mimosa

Pi 0.0744
MarkerSeek score 81.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
458
variable_sites
61
pi
0.0744
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAAGGGGTATTGGGTAGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.7 58.0 591-651 bp 0.75 79.1
rpl32-trnL-UAG_p2 TAAACCACCAAGTAAGCGGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 1005 bp 0.25 48.4
rpl32-trnL-UAG_p3 TAAACCACCAAGTAAGCGGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 1006 bp 0.25 48.4
rpl32-trnL-UAG_p4 TAAACCACCAAGTAAGCGGG GCTGCATAGCCCAATACCAT 50.0 50.0 57.8 58.1 1594 bp 0.25 47.8
rpl32-trnL-UAG_p5 TAAACCACCAAGTAAGCGGG GAAGTAGCAAAGCCTTGGGT 50.0 50.0 57.8 58.1 1492 bp 0.25 47.8