Hotspot

ndhF-rpl32

SSC · 69939-70930 · 992 bp

Back to Mimosa

Pi 0.0687
MarkerSeek score 80.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
992
variable_sites
105
pi
0.0687
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.82
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGGAATTGGGCAGACTTAG AGCGTGTCTACCGATTTCAC 55.0 50.0 57.9 58.0 2318-2485 bp 1.0 90.3
ndhF-rpl32_p2 GGGGAATTGGGCAGACTTAG CAGCGTGTCTACCGATTTCA 55.0 50.0 57.9 58.0 2319-2486 bp 1.0 90.3
ndhF-rpl32_p3 AATAACAGGAAGGCGCACAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2284-2348 bp 0.75 78.5
ndhF-rpl32_p4 ATAACAGGAAGGCGCACAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2283-2347 bp 0.75 78.5
ndhF-rpl32_p5 AAATAACAGGAAGGCGCACA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2285-2349 bp 0.5 67.5