Hotspot

rps8-rpl14

LSC · 36960-37661 · 702 bp

Back to Mimosa

Pi 0.148
MarkerSeek score 73.00
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
702
variable_sites
83
pi
0.148
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.46
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CCGGAAACATGACCACCTAG CGTATGGGGTCAGGAAAAGG 55.0 55.0 58.0 58.0 1583-2033 bp 0.75 79.7
rps8-rpl14_p2 CCGGAAACATGACCACCTAG TGGGGTCAGGAAAAGGATCT 55.0 50.0 58.0 57.9 1579-2029 bp 0.75 79.5
rps8-rpl14_p3 CCGGAAACATGACCACCTAG AGCCGGTTACAGTAAGACCT 55.0 50.0 58.0 58.1 1612-2062 bp 0.75 79.3
rps8-rpl14_p4 GTCGAATCCTTCCGGAAACA TTTACTTCGCCCTTTGCACT 50.0 45.0 57.9 58.0 1288-1783 bp 0.5 67.3
rps8-rpl14_p5 CCGGAAACATGACCACCTAG TTTACTTCGCCCTTTGCACT 55.0 45.0 58.0 58.0 1772 bp 0.25 49.6