Hotspot

trnP-UGG-psaJ

LSC · 22298-22732 · 435 bp

Back to Mimosa

Pi 0.0651
MarkerSeek score 83.20
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
435
variable_sites
48
pi
0.0651
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 537-662 bp 1.0 88.3
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 536-661 bp 1.0 88.3
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 548-673 bp 1.0 88.1
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 547-672 bp 1.0 88.1
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 550-675 bp 1.0 88.1