Hotspot

petA-psbJ

LSC · 17947-19305 · 1359 bp

Back to Mimosa

Pi 0.0806
MarkerSeek score 93.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1359
variable_sites
178
pi
0.0806
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
93.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GTCTCTTGTTCTTCGTGGCA ATTCCGGATTGGGTTCATCC 50.0 50.0 58.1 57.6 1458-1579 bp 1.0 88.0
petA-psbJ_p2 CGTGTCCAAGGTCTCTTGTT ATTCCGGATTGGGTTCATCC 50.0 50.0 57.8 57.6 1468-1589 bp 1.0 87.2
petA-psbJ_p3 TGTTCTTCGTGGCATCTGTT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.7 57.6 1452-1573 bp 1.0 87.0
petA-psbJ_p4 TTGTTCTTCGTGGCATCTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.7 57.6 1453-1574 bp 1.0 87.0
petA-psbJ_p5 CGTTACGTGTCCAAGGTCTC ATTCCGGATTGGGTTCATCC 55.0 50.0 58.3 57.6 1473-1594 bp 1.0 86.6