Hotspot

accD-psaI

LSC · 13951-14732 · 782 bp

Back to Mimosa

Pi 0.15
MarkerSeek score 76.30
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
782
variable_sites
125
pi
0.15
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.58
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GCTCCATGCTTTCTTTCCTT AGAGGGTAAGTTGCTGAGAGT 45.0 47.6 56.3 58.1 529-843 bp 1.0 69.5
accD-psaI_p2 GCTCCATGCTTTCTTTCCTT GGGTAAGTTGCTGAGAGTTGT 45.0 47.6 56.3 57.9 526-840 bp 1.0 69.3
accD-psaI_p3 GCTCCATGCTTTCTTTCCTTT AGAGGGTAAGTTGCTGAGAGT 42.9 47.6 57.1 58.1 529-843 bp 1.0 67.6
accD-psaI_p4 GCTCCATGCTTTCTTTCCTTT GGGTAAGTTGCTGAGAGTTGT 42.9 47.6 57.1 57.9 526-840 bp 1.0 67.4
accD-psaI_p5 GCTCCATGCTTTCTTTCCTT AGGGTAAGTTGCTGAGAGTTG 45.0 47.6 56.3 57.6 527-841 bp 1.0 67.1