Hotspot

ndhC-trnV-UAC

LSC · 4730-6043 · 1314 bp

Back to Mimosa

Pi 0.11
MarkerSeek score 78.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1314
variable_sites
181
pi
0.11
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.71
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTCGA AGAAGGTCTACGGTTCGAGT 37.5 50.0 58.0 57.8 1134-1419 bp 1.0 58.3
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTCG AGAAGGTCTACGGTTCGAGT 39.1 50.0 56.7 57.8 1134-1419 bp 1.0 55.8
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTCGA GAAGGTCTACGGTTCGAGTC 37.5 55.0 58.0 57.5 1133-1418 bp 1.0 55.5
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTCGA GTTCGAGTCCGTATAGCCCT 37.5 55.0 58.0 58.7 1122-1407 bp 1.0 54.4
ndhC-trnV-UAC_p5 GGGGCTAAAATTCCAGAAAT AGAAGGTCTACGGTTCGAGT 40.0 50.0 53.5 57.8 1160-1445 bp 1.0 54.0