Hotspot

trnF-GAA-ndhJ

LSC · 2264-3016 · 753 bp

Back to Mimosa

Pi 0.0503
MarkerSeek score 84.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
753
variable_sites
68
pi
0.0503
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 TTCAAATCTGGTTCCTGGCA GTTGGATAGGATGGCCCTTG 45.0 55.0 57.3 58.0 818-844 bp 1.0 85.1
trnF-GAA-ndhJ_p2 TTCAAATCTGGTTCCTGGCA AGTTGGATAGGATGGCCCTT 45.0 50.0 57.3 58.1 819-845 bp 1.0 84.5
trnF-GAA-ndhJ_p3 TTCAAATCTGGTTCCTGGCA ATGGCCCTTGCGTAAAGATT 45.0 45.0 57.3 57.9 808-834 bp 1.0 84.1
trnF-GAA-ndhJ_p4 TTCAAATCTGGTTCCTGGCA GATGGCCCTTGCGTAAAGAT 45.0 50.0 57.3 58.3 809-835 bp 1.0 82.7
trnF-GAA-ndhJ_p5 TTCAAATCTGGTTCCTGGCA ATGCCCGAAAGTTGGATAGG 45.0 50.0 57.3 57.7 828-854 bp 1.0 82.3