Hotspot

rps15-ycf1

SSC · 123291-123684 · 394 bp

Back to Millettia

Pi 0.0385

MarkerSeek score 65.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 15
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACTGATCCCGTGTTTTCT	TCCTCGGTTGAAAATTCGTT	42.9	40.0	57.5	55.6	480-484 bp	1.0	52.1
rps15-ycf1_p2	TCAACTGATCCCGTGTTTTCT	ATCCTCGGTTGAAAATTCGT	42.9	40.0	57.5	55.4	481-485 bp	1.0	50.2
rps15-ycf1_p3	ACTGATCCCGTGTTTTCTTCT	TCCTCGGTTGAAAATTCGTT	42.9	40.0	57.2	55.6	477-481 bp	1.0	49.8
rps15-ycf1_p4	TCAACTGATCCCGTGTTTTCT	ATCCTCGGTTGAAAATTCGTT	42.9	38.1	57.5	56.2	481-485 bp	1.0	48.6
rps15-ycf1_p5	ACTGATCCCGTGTTTTCTTCT	ATCCTCGGTTGAAAATTCGT	42.9	40.0	57.2	55.4	478-482 bp	1.0	47.9