Hotspot

atpF-atpA

LSC · 50124-50233 · 110 bp

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Pi 0.0463

MarkerSeek score 71.70

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 110
variable_sites: 5
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	TGTTTGGGGCGATGAAAGAA	CCGTCGCCTACTTGAAGTAC	45.0	55.0	58.0	58.1	253-255 bp	1.0	81.4
atpF-atpA_p2	TGTTTGGGGCGATGAAAGAA	GTCGCCTACTTGAAGTACGG	45.0	55.0	58.0	58.1	251-253 bp	1.0	81.4
atpF-atpA_p3	ATGTTTGGGGCGATGAAAGA	CCGTCGCCTACTTGAAGTAC	45.0	55.0	57.8	58.1	254-256 bp	1.0	79.7
atpF-atpA_p4	ATGTTTGGGGCGATGAAAGA	GTCGCCTACTTGAAGTACGG	45.0	55.0	57.8	58.1	252-254 bp	1.0	79.7
atpF-atpA_p5	CATGTTTGGGGCGATGAAAG	GTCGCCTACTTGAAGTACGG	50.0	55.0	57.7	58.1	253-255 bp	1.0	79.2