Hotspot

atpH-atpF

LSC · 48266-48831 · 566 bp

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Pi 0.0196

MarkerSeek score 58.50

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 11
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TGGGCTGGTTGTAGCATTAG	AGCCCAAGCAAAGGAAAGAA	50.0	45.0	57.6	57.9	646-652 bp	1.0	78.1
atpH-atpF_p2	TGGGCTGGTTGTAGCATTAG	CAGTAGCCCAAGCAAAGGAA	50.0	50.0	57.6	58.1	650-656 bp	1.0	78.0
atpH-atpF_p3	AGGCAGAGGGAAAAATACGG	AGCCCAAGCAAAGGAAAGAA	50.0	45.0	57.6	57.9	713-719 bp	1.0	77.8
atpH-atpF_p4	AGGCAGAGGGAAAAATACGG	CAGTAGCCCAAGCAAAGGAA	50.0	50.0	57.6	58.1	717-723 bp	1.0	77.8
atpH-atpF_p5	CCAGAGGCAGAGGGAAAAAT	AGCCCAAGCAAAGGAAAGAA	50.0	45.0	57.5	57.9	717-723 bp	1.0	77.0