Hotspot

atpI-atpH

LSC · 46824-48019 · 1196 bp

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Pi 0.0156

MarkerSeek score 57.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1196
variable_sites: 18
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GAATATGGTCCGTGGGTTCC	ATAACGGAAGCGGCAGAAAT	55.0	45.0	58.0	58.0	1670-1705 bp	1.0	82.3
atpI-atpH_p2	TCTGCTGTGGAAGTAGGTCA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.0	58.0	1886-1921 bp	1.0	82.2
atpI-atpH_p3	GAATATGGTCCGTGGGTTCC	CAAGAGCAGCAAAGCAAGTC	55.0	50.0	58.0	58.0	1950-1989 bp	1.0	82.2
atpI-atpH_p4	TCTGCTGTGGAAGTAGGTCA	CAAGAGCAGCAAAGCAAGTC	50.0	50.0	58.0	58.0	2166-2205 bp	1.0	82.1
atpI-atpH_p5	GGCAAATAGGGGGTTTCCAA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.1	58.0	1855-1890 bp	1.0	81.9