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psbM-petN

LSC · 31620-32493 · 874 bp

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Pi 0.0127

MarkerSeek score 60.60

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 11
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ACGGTAAGTCAAAGTGGTGAC	TCTTGCTTGGGCTGCTTTAA	47.6	45.0	58.2	58.0	973-974 bp	1.0	72.1
psbM-petN_p2	ACGGTAAGTCAAAGTGGTGA	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	56.7	58.0	973-974 bp	1.0	71.2
psbM-petN_p3	ACGGTAAGTCAAAGTGGTGAC	AGTAAGTCTTGCTTGGGCTG	47.6	50.0	58.2	57.8	979-980 bp	1.0	70.6
psbM-petN_p4	ACGGTAAGTCAAAGTGGTGA	AGTAAGTCTTGCTTGGGCTG	45.0	50.0	56.7	57.8	979-980 bp	1.0	69.7
psbM-petN_p5	AACGGTAAGTCAAAGTGGTGA	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.5	58.0	974-975 bp	1.0	69.1