Hotspot

trnT-rps4

LSC · 15030-15345 · 316 bp

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Pi 0.0164

MarkerSeek score 50.90

Primer available yes

Label trnT-rps4

Feature Metadata

label_name: trnT-rps4
feature_type: igs
region: LSC
length_bp: 316
variable_sites: 5
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-rps4_p1	GTTATTGAGCCCGCTTAGCT	AGGTCCTCTGTAACGTGACA	50.0	50.0	58.0	58.0	412-418 bp	1.0	83.0
trnT-rps4_p2	GTTATTGAGCCCGCTTAGCT	AGCGCGATTGGTTTCTAAGT	50.0	45.0	58.0	57.9	503-509 bp	1.0	81.9
trnT-rps4_p3	GTTATTGAGCCCGCTTAGCT	CGGGCTTGAGGAATAGTCAC	50.0	55.0	58.0	58.1	714-720 bp	1.0	81.4
trnT-rps4_p4	GTTATTGAGCCCGCTTAGCT	AACTTTTCCGGCGATACGAA	50.0	45.0	58.0	57.9	616-622 bp	1.0	81.4
trnT-rps4_p5	GTTATTGAGCCCGCTTAGCT	TCACAGCCATACCCAACCTA	50.0	50.0	58.0	58.0	704 bp	0.5	59.3