Hotspot

atpF

LSC · 12787-14124 · 1338 bp

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Pi 0

MarkerSeek score 59.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1338
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCATCGGCTCGAATGGTT	AGACTAAGAAAGAGGAGATCA	45.0	38.1	57.9	52.7	1438-1445 bp	1.0	40.4
atpF_p2	TTTCATCGGCTCGAATGGTT	AAGACTAAGAAAGAGGAGATCA	45.0	36.4	57.9	53.6	1439-1446 bp	1.0	40.4
atpF_p3	TCATCGGCTCGAATGGTTAC	AGACTAAGAAAGAGGAGATCA	50.0	38.1	57.8	52.7	1436-1443 bp	1.0	40.4
atpF_p4	TCATCGGCTCGAATGGTTAC	AAGACTAAGAAAGAGGAGATCA	50.0	36.4	57.8	53.6	1437-1444 bp	1.0	40.4
atpF_p5	GGCTCGAATGGTTACCATGA	AGACTAAGAAAGAGGAGATCA	50.0	38.1	57.7	52.7	1431-1438 bp	1.0	40.4