Hotspot

ndhF-rpl32

SSC · 116734-118111 · 1378 bp

Back to Milicia

Pi 0.00145

MarkerSeek score 59.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1378
variable_sites: 2
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACATACGGCGAAGACTTT	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.3	57.8	1592-1594 bp	1.0	76.6
ndhF-rpl32_p2	ACGGCGAAGACTTTTTGTTG	AAAAGCTTTCAATGCTGCCC	45.0	45.0	57.5	57.8	1586-1588 bp	1.0	75.1
ndhF-rpl32_p3	CGAAGACTTTTTGTTGCCGT	AAAAGCTTTCAATGCTGCCC	45.0	45.0	57.5	57.8	1582-1584 bp	1.0	75.1
ndhF-rpl32_p4	GGCGAAGACTTTTTGTTGCC	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.5	57.8	1584-1586 bp	1.0	74.7
ndhF-rpl32_p5	GACTTTTTGTTGCCGTTGGA	AAAAGCTTTCAATGCTGCCC	45.0	45.0	57.4	57.8	1578-1580 bp	1.0	74.2