Hotspot

trnP-UGG-psaJ

LSC · 70915-71361 · 447 bp

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Pi 0.00227

MarkerSeek score 58.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 1
pi: 0.00227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	604-611 bp	1.0	80.2
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	603-610 bp	1.0	80.1
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	615-622 bp	1.0	80.1
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	614-621 bp	1.0	80.1
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	617-624 bp	1.0	80.1