Hotspot

trnE-UUC-trnT-GGU

LSC · 33298-33978 · 681 bp

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Pi 0.00587
MarkerSeek score 62.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
681
variable_sites
4
pi
0.00587
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 768-773 bp 1.0 79.2
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 766-771 bp 1.0 79.2
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 822-827 bp 1.0 79.1
trnE-UUC-trnT-GGU_p4 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 747-752 bp 1.0 78.8
trnE-UUC-trnT-GGU_p5 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 745-750 bp 1.0 78.8