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trnK-UUU-rps16

LSC · 4510-5539 · 1030 bp

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Pi 0.000976

MarkerSeek score 59.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 1
pi: 0.000976
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	TATTTCAAAGAAGGCGGGGG	50.0	50.0	57.9	57.9	1206-1211 bp	1.0	78.8
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	57.9	57.9	1205-1210 bp	1.0	78.8
trnK-UUU-rps16_p3	AGTCGCGGTCTTACAAACTC	AGAAGGCGGGGGTTTTTATG	50.0	50.0	57.9	58.2	1198-1203 bp	1.0	78.7
trnK-UUU-rps16_p4	GTCGCGGTCTTACAAACTCT	AGAAGGCGGGGGTTTTTATG	50.0	50.0	57.9	58.2	1197-1202 bp	1.0	78.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1122-1127 bp	1.0	78.0