Hotspot

rrn23

IRa · 129397-132208 · 2812 bp

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Pi 0.00648

MarkerSeek score 56.20

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: IRa
length_bp: 2812
variable_sites: 142
pi: 0.00648
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	AAACTTCTGTCGCTCCATCC	TCCATCCATTTAGCAGCACC	50.0	50.0	57.9	57.9	2870-2951 bp	1.0	86.6
rrn23_p2	AAACTTCTGTCGCTCCATCC	CCATCCATTTAGCAGCACCT	50.0	50.0	57.9	57.9	2869-2950 bp	1.0	86.6
rrn23_p3	AAACTTCTGTCGCTCCATCC	CGGCGGTTCGATCTATGATT	50.0	50.0	57.9	57.9	2914-2995 bp	1.0	86.5
rrn23_p4	GCCGTGACCTTCTCTTGAAT	TCCATCCATTTAGCAGCACC	50.0	50.0	57.9	57.9	2895-2976 bp	0.9375	80.8
rrn23_p5	GCCGTGACCTTCTCTTGAAT	CCATCCATTTAGCAGCACCT	50.0	50.0	57.9	57.9	2894-2975 bp	0.9375	80.8