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rpl32-ndhF

SSC · 123767-124774 · 1008 bp

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Pi 0.0112

MarkerSeek score 73.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1008
variable_sites: 61
pi: 0.0112
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCCCATATCCTTTCCTTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1109-1211 bp	1.0	85.0
rpl32-ndhF_p2	CGCCCCATATCCTTTCCTTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1107-1209 bp	1.0	85.0
rpl32-ndhF_p3	CGCCCCATATCCTTTCCTTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1108-1210 bp	1.0	85.0
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1127-1229 bp	1.0	84.8
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.6	58.0	1125-1227 bp	1.0	84.7