Hotspot

trnL-UAG-rpl32

SSC · 122858-123601 · 744 bp

Back to Mikania

Pi 0.0147

MarkerSeek score 57.90

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 744
variable_sites: 48
pi: 0.0147
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	AAAGGAAAGGATATGGGGCG	50.0	50.0	58.0	57.6	860-893 bp	1.0	83.4
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	AAAGGAAAGGATATGGGGCG	50.0	50.0	58.0	57.6	861-894 bp	1.0	83.4
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	842-875 bp	1.0	83.3
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	843-876 bp	1.0	83.2
trnL-UAG-rpl32_p5	GAAGTAGCAAAGCCTTGGGT	AAAGGAAAGGATATGGGGCG	50.0	50.0	58.1	57.6	1362-1395 bp	1.0	80.8