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ndhC-trnV-UAC

LSC · 49846-51012 · 1167 bp

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Pi 0.00686

MarkerSeek score 71.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1167
variable_sites: 39
pi: 0.00686
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1275-1359 bp	1.0	83.8
ndhC-trnV-UAC_p2	ACTCGGAGCTAAAAATCCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1266-1350 bp	1.0	82.4
ndhC-trnV-UAC_p3	TTCTCCGGTCCTTTGCTACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1283-1367 bp	1.0	82.0
ndhC-trnV-UAC_p4	TTTCTCCGGTCCTTTGCTAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1284-1368 bp	1.0	81.1
ndhC-trnV-UAC_p5	TCCTTTGCTACTCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1274-1358 bp	1.0	81.0