Hotspot

ycf3-trnS-GGA

LSC · 44074-44988 · 915 bp

Back to Mikania

Pi 0.00665
MarkerSeek score 64.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
915
variable_sites
27
pi
0.00665
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA CAACCACTCAGCCATCTCTC 45.0 55.0 56.4 58.0 805-1051 bp 1.0 73.4
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA CAACCACTCAGCCATCTCTC 45.0 55.0 56.4 58.0 806-1052 bp 1.0 73.4
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA ATGCTACGCTTTCAACCACT 45.0 45.0 56.4 57.8 817-1063 bp 1.0 72.1
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA ATGCTACGCTTTCAACCACT 45.0 45.0 56.4 57.8 818-1064 bp 1.0 72.1
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 860-1106 bp 1.0 71.7