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trnK-UUU-rps16

LSC · 4382-5200 · 819 bp

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Pi 0.0102

MarkerSeek score 60.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 43
pi: 0.0102
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	946-974 bp	1.0	80.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	945-973 bp	1.0	80.9
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	955-983 bp	1.0	80.1
trnK-UUU-rps16_p4	TACCGTTGAGTTAGCAACCC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.5	58.3	932-960 bp	1.0	78.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AGGCAGAGGTTTTTAAGGCA	50.0	45.0	58.2	57.3	893-921 bp	1.0	78.2