Hotspot

ycf1

SSC · 119738-125074 · 5337 bp

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Pi 0.0146

MarkerSeek score 46.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5337
variable_sites: 77
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAGATTCGGTATTGGACGG	TGGATACAGATCGATACCAA	55.0	40.0	58.1	52.9	193-214 bp	1.0	42.8
ycf1_p2	CAGATTCGGTATTGGACGGT	TGGATACAGATCGATACCAA	50.0	40.0	57.4	52.9	192-213 bp	1.0	42.8
ycf1_p3	CAGATTCGGTATTGGACGGTT	TGGATACAGATCGATACCAA	47.6	40.0	58.1	52.9	192-213 bp	1.0	42.8
ycf1_p4	TCCAGATTCGGTATTGGACG	TGGATACAGATCGATACCAA	50.0	40.0	57.1	52.9	194-215 bp	1.0	42.7
ycf1_p5	ACCAAAGTTCCAGATTCGGT	TGGATACAGATCGATACCAA	45.0	40.0	57.0	52.9	202-223 bp	1.0	42.6