Hotspot

rps8

LSC · 77289-77696 · 408 bp

Back to Microula

Pi 0.0196

MarkerSeek score 55.50

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 408
variable_sites: 8
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	CAGACCCAACTTGGATTTGGA	TCCAATTTTGAAACATCAAGAA	47.6	27.3	58.1	52.7	474 bp	0.5	20.0
rps8_p2	TCAGACCCAACTTGGATTTGG	TCCAATTTTGAAACATCAAGAA	47.6	27.3	58.1	52.7	475 bp	0.5	20.0
rps8_p3	AGACAACTCGCTCCTTTTCT	TCCAATTTTGAAACATCAAGAA	45.0	27.3	56.8	52.7	519 bp	0.5	20.0
rps8_p4	CGGTATTAGACAACTCGCTCC	TCCAATTTTGAAACATCAAGAA	52.4	27.3	58.3	52.7	526 bp	0.5	20.0
rps8_p5	CAGACCCAACTTGGATTTGGA	TCCAATTTTGAAACATCAAGAAA	47.6	26.1	58.1	53.6	474 bp	0.5	20.0