Hotspot

ndhD-psaC

SSC · 113207-113323 · 117 bp

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Pi 0.0431

MarkerSeek score 68.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 117
variable_sites: 5
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GGGAGCCAGTGAATATTGGG	AGGATCTGAAACAACTCGCA	55.0	45.0	58.0	57.2	214-215 bp	1.0	75.4
ndhD-psaC_p2	GGGAGCCAGTGAATATTGGG	GGATCTGAAACAACTCGCAG	55.0	50.0	58.0	56.8	213-214 bp	1.0	72.8
ndhD-psaC_p3	GGGAGCCAGTGAATATTGGG	AGGATCTGAAACAACTCGCAG	55.0	47.6	58.0	58.3	214-215 bp	1.0	71.7
ndhD-psaC_p4	GGGAGCCAGTGAATATTGGG	ACGGATTTCTTGAGTGTTCGA	55.0	42.9	58.0	57.6	243-244 bp	1.0	70.8
ndhD-psaC_p5	GGGAGCCAGTGAATATTGGG	CGCAGTATGGGTCTAGCTTA	55.0	50.0	58.0	56.5	198-199 bp	1.0	70.6