Hotspot

ccsA-ndhD

SSC · 111521-111703 · 183 bp

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Pi 0.0533

MarkerSeek score 67.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 183
variable_sites: 8
pi: 0.0533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGCTACACAGTTACGGTTT	TTAACATCTCTCGGCCAACG	50.0	50.0	57.8	58.0	827-852 bp	1.0	83.5
ccsA-ndhD_p2	GGGCTACACAGTTACGGTTT	CTCTCGGCCAACGGAATTTA	50.0	50.0	57.8	58.0	820-845 bp	1.0	83.5
ccsA-ndhD_p3	TGCAGAGTGTAAATTCCGCA	TTAACATCTCTCGGCCAACG	45.0	50.0	57.8	58.0	913-938 bp	1.0	83.0
ccsA-ndhD_p4	CGAAGCATGGGGATCGTATT	TTAACATCTCTCGGCCAACG	50.0	50.0	57.8	58.0	1017-1042 bp	1.0	82.6
ccsA-ndhD_p5	CGAAGCATGGGGATCGTATT	CTCTCGGCCAACGGAATTTA	50.0	50.0	57.8	58.0	1010-1035 bp	1.0	82.6