Hotspot

rps8-rpl14

LSC · 77697-77856 · 160 bp

Back to Microula

Pi 0.0566

MarkerSeek score 82.60

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 9
pi: 0.0566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	GTTTCATTGGCTCCTGAGGT	50.0	50.0	57.7	57.8	207-208 bp	1.0	80.1
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	CGCTCGGGAATTGAGACAAT	50.0	50.0	57.7	58.3	244-245 bp	1.0	78.4
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	AATTTTTGGTGCCATCGCTC	50.0	45.0	57.7	57.6	259-260 bp	1.0	78.0
rps8-rpl14_p4	AGCAATAGTGTCCCTACCCA	GAATTTTTGGTGCCATCGCT	50.0	45.0	57.7	57.6	260-261 bp	1.0	78.0
rps8-rpl14_p5	AGCAATAGTGTCCCTACCCA	AGTTTCATTGGCTCCTGAGG	50.0	50.0	57.7	57.5	208-209 bp	1.0	77.7