Hotspot

psbT-psbN

LSC · 71351-71419 · 69 bp

Back to Microula

Pi 0.029

MarkerSeek score 69.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 2
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTACAA	GGGTACGCCTTATATACCGC	45.0	55.0	57.5	57.7	182-187 bp	1.0	76.5
psbT-psbN_p2	TTTTTCGAGAACCGCCTACA	GGGTACGCCTTATATACCGC	45.0	55.0	57.5	57.7	183-188 bp	1.0	76.5
psbT-psbN_p3	TCGAGAACCGCCTACAATTT	GGGTACGCCTTATATACCGC	45.0	55.0	57.2	57.7	179-184 bp	1.0	74.8
psbT-psbN_p4	TTCGAGAACCGCCTACAATT	GGGTACGCCTTATATACCGC	45.0	55.0	57.2	57.7	180-185 bp	1.0	74.8
psbT-psbN_p5	TTTCGAGAACCGCCTACAAT	GGGTACGCCTTATATACCGC	45.0	55.0	57.2	57.7	181-186 bp	1.0	74.7