Hotspot

trnP-UGG-psaJ

LSC · 63828-64245 · 418 bp

Back to Microula

Pi 0.0315

MarkerSeek score 67.30

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 13
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	547-569 bp	1.0	81.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	546-568 bp	1.0	81.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	558-580 bp	1.0	81.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	557-579 bp	1.0	81.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	560-582 bp	1.0	81.6