Hotspot

atpB-rbcL

LSC · 53123-53822 · 700 bp

Back to Microula

Pi 0.0344

MarkerSeek score 72.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 24
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATACGCCCCGGGTTATTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	818-834 bp	1.0	74.2
atpB-rbcL_p2	CCCCAGAACCAGAAGGAGTA	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	783-799 bp	1.0	74.2
atpB-rbcL_p3	GATACGCCCCGGGTTATTTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	817-833 bp	1.0	74.0
atpB-rbcL_p4	TTTTAAGCGTGGAAACCCCA	CACCAGCTTTGAATCCAACAC	45.0	47.6	57.9	58.0	798-814 bp	1.0	74.0
atpB-rbcL_p5	CCCCAGAACCAGAAGGAGTA	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	782-798 bp	1.0	74.0