Hotspot

rpoC1-rpoB

LSC · 23050-23081 · 32 bp

Back to Microula

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 32
variable_sites: 1
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGGCCCAAGCACTTATTTGT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	225 bp	1.0	75.9
rpoC1-rpoB_p2	TGTGGCCCAAGCACTTATTT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	227 bp	1.0	75.9
rpoC1-rpoB_p3	TTGTGGCCCAAGCACTTATT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	228 bp	1.0	75.9
rpoC1-rpoB_p4	TTTGTGGCCCAAGCACTTAT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	229 bp	1.0	75.9
rpoC1-rpoB_p5	AATTTTTGTGGCCCAAGCAC	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	233 bp	1.0	75.7