Hotspot

atpA-atpF

LSC · 11330-11388 · 59 bp

Back to Microula

Pi 0.105

MarkerSeek score 78.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 6
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TTGGGGGCGATGAAAGAAAT	55.0	45.0	58.1	57.8	200-202 bp	1.0	80.6
atpA-atpF_p2	ACTAATTTCGTCGGCTCGAA	TTGGGGGCGATGAAAGAAAT	45.0	45.0	57.4	57.8	117-119 bp	1.0	78.4
atpA-atpF_p3	CGTCGCCTACTTGAAGTACA	TTGGGGGCGATGAAAGAAAT	50.0	45.0	57.4	57.8	199-201 bp	1.0	76.4
atpA-atpF_p4	AATTTCGTCGGCTCGAATGG	TTGGGGGCGATGAAAGAAAT	50.0	45.0	59.0	57.8	114-116 bp	1.0	75.8
atpA-atpF_p5	CCGTCGCCTACTTGAAGTAC	TGGGGGCGATGAAAGAAATA	55.0	45.0	58.1	56.9	199-201 bp	1.0	73.5