Hotspot

trnG-UCC-trnR-UCU

LSC · 9469-9670 · 202 bp

Back to Microula

Pi 0.0432

MarkerSeek score 70.70

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 7
pi: 0.0432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.0	56.7	292-329 bp	1.0	75.5
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGAAACAAATTGCGTCCAA	55.0	40.0	58.1	56.7	295-332 bp	1.0	75.3
trnG-UCC-trnR-UCU_p3	CCCTAGCCTTCCAAGCTAAC	TGGAAACAAATTGCGTCCAA	55.0	40.0	57.7	56.7	296-333 bp	1.0	73.1
trnG-UCC-trnR-UCU_p4	CTAACGATGCGGGTTCGATT	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.4	56.7	281-318 bp	1.0	72.4
trnG-UCC-trnR-UCU_p5	TAACGATGCGGGTTCGATTC	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.4	56.7	280-317 bp	1.0	72.3