Hotspot

rps16-trnQ-UUG

LSC · 5808-6689 · 882 bp

Back to Microula

Pi 0.0249

MarkerSeek score 68.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 20
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	935-962 bp	1.0	85.0
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	TTGACTAGAAATGGGGCGTG	50.0	50.0	58.0	57.9	994-1021 bp	1.0	84.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	928-955 bp	1.0	84.0
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTGACTAGAAATGGGGCGTG	45.0	50.0	58.1	57.9	987-1014 bp	1.0	83.6
rps16-trnQ-UUG_p5	CCACAGCGGATCTTGTCTTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	949-976 bp	1.0	83.6