Hotspot

rpl14

LSC · 84975-85343 · 369 bp

Back to Microtropis

Pi 0.00813

MarkerSeek score 44.60

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 3
pi: 0.00813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	TGTCAGCAAGAGTGTCCCTA	CCCACCCAAAGAAAAGAGCT	50.0	50.0	58.0	58.0	751-777 bp	1.0	80.9
rpl14_p2	ATTACAATCCCCATTCCGCC	CCCACCCAAAGAAAAGAGCT	50.0	50.0	58.0	58.0	1037-1063 bp	1.0	80.7
rpl14_p3	ATTACAATCCCCATTCCGCC	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	1376-1402 bp	1.0	80.5
rpl14_p4	TGTCAGCAAGAGTGTCCCTA	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	1090-1116 bp	1.0	80.5
rpl14_p5	ATTACAATCCCCATTCCGCC	GTTAAACCGGGGCGAATACT	50.0	50.0	58.0	58.0	1180-1206 bp	1.0	80.5