Hotspot

ccsA

SSC · 117081-118073 · 993 bp

Back to Microtropis

Pi 0.00504

MarkerSeek score 49.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 993
variable_sites: 5
pi: 0.00504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAATAGTTCCTAGAATGAATTAAATTC	ACACTTATATGCTGTCCTGTGT	25.9	40.9	53.2	57.2	1088 bp	1.0	40.4
ccsA_p2	GAATAGTTCCTAGAATGAATTAAATTC	TCAAATGGTTCTCGATGGTT	25.9	40.0	53.2	55.0	1127 bp	1.0	40.4
ccsA_p3	GAATAGTTCCTAGAATGAATTAAATTC	TTCAAATGGTTCTCGATGGT	25.9	40.0	53.2	55.0	1128 bp	1.0	40.4
ccsA_p4	GAATAGTTCCTAGAATGAATTAAATTC	ACACTTATATGCTGTCCTGTGTT	25.9	39.1	53.2	57.8	1088 bp	1.0	40.4
ccsA_p5	GAATAGTTCCTAGAATGAATTAAATTC	TCAAATGGTTCTCGATGGTTT	25.9	38.1	53.2	55.8	1127 bp	1.0	40.4