Hotspot

trnN-GUU-trnR-ACG

IRa · 132586-133201 · 616 bp

Back to Microtropis

Pi 0.00658
MarkerSeek score 63.80
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
616
variable_sites
4
pi
0.00658
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 749-757 bp 1.0 79.5
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 55.0 55.0 58.0 57.7 786-794 bp 1.0 78.9
trnN-GUU-trnR-ACG_p3 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.1 57.8 790-798 bp 1.0 78.7
trnN-GUU-trnR-ACG_p4 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 713-721 bp 1.0 78.4
trnN-GUU-trnR-ACG_p5 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 700-708 bp 1.0 78.4