Hotspot

ccsA-ndhD

SSC · 118074-118285 · 212 bp

Back to Microtropis

Pi 0.0433

MarkerSeek score 78.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 212
variable_sites: 9
pi: 0.0433
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTAAGTTCCGCAATTGTGGC	TCAGTTGACAAGGTTGAGGC	50.0	50.0	58.0	58.0	367-371 bp	1.0	82.4
ccsA-ndhD_p2	GTAAGTTCCGCAATTGTGGC	TCGGCATTTATCCGGACTTG	50.0	50.0	58.0	58.1	399-403 bp	1.0	82.0
ccsA-ndhD_p3	GTAAGTTCCGCAATTGTGGC	GGCATTTATCCGGACTTGGT	50.0	50.0	58.0	57.9	397-401 bp	1.0	82.0
ccsA-ndhD_p4	TAAGTTCCGCAATTGTGGCT	TCAGTTGACAAGGTTGAGGC	45.0	50.0	58.1	58.0	366-370 bp	1.0	81.8
ccsA-ndhD_p5	TAAGTTCCGCAATTGTGGCT	TCGGCATTTATCCGGACTTG	45.0	50.0	58.1	58.1	398-402 bp	1.0	81.4