Hotspot

ndhF-rpl32

SSC · 115643-116247 · 605 bp

Back to Microtropis

Pi 0.00698

MarkerSeek score 67.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 605
variable_sites: 4
pi: 0.00698
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAACATACGGCGAAGGTTTT	TCAATGCTGCCCAATATCCC	45.0	50.0	57.0	58.0	781-813 bp	1.0	75.8
ndhF-rpl32_p2	CAACATACGGCGAAGGTTTT	CAATGCTGCCCAATATCCCT	45.0	50.0	57.0	58.0	780-812 bp	1.0	75.7
ndhF-rpl32_p3	AAAGTAGAAGACCTCCCCCT	TCAATGCTGCCCAATATCCC	50.0	50.0	57.0	58.0	746-778 bp	1.0	75.4
ndhF-rpl32_p4	AAAGTAGAAGACCTCCCCCT	CAATGCTGCCCAATATCCCT	50.0	50.0	57.0	58.0	745-777 bp	1.0	75.4
ndhF-rpl32_p5	CAACATACGGCGAAGGTTTT	TGCTGCCCAATATCCCTTTC	45.0	50.0	57.0	57.9	777-809 bp	1.0	75.2